Professor visitante do PPGBMC, Pablo Trindade, palestra no no Neuroscience 2019 em Chicago
No dia 23/10/2019, o professor visitante do PPGBMC Pablo Trindade, fez apresentou a palestra sobre o tema Characterization of functional astrogliosis using human induced pluripotent stem cells-derived astrocytes no Neuroscience 2019 em Chicago, EUA. Essa participação revela a excelência da produção científica do PPGBMC. Abaixo segue o resumo do trabalho aprovado e apresentado.
Authors
*P. TRINDADE , E. C. LOIOLA , P. F. LEDUR , J. D. SALERNO , L. R. Q. SOUZA , P. L. CARDOZO , S. DEVALLE , I. M. ORNELAS , F. M. RIBEIRO , A. M. VENTURA , D. P. GELAIN , L. O. PORCIÚNCULA , S. K. REHEN ; UNIRIO, Rio de Janeiro, Brazil; IDOR, Rio de Janeiro, Brazil; UFRJ, Rio de Janeiro, Brazil; UFMG, Belo Horizonte, Brazil; UFF, Niterói, Brazil; UFRGS, Porto Alegre, Brazil
Abstract
Astrogliosis has been implicated in several neurodegenerative and neuropsychiatric disorders. However, most mechanisms underlying astrogliosis were described using animal models, which fail to fully reproduce the complexity of human cell signaling. Here, we report a model to study astrogliosis in a dish using human induced pluripotent stem cells (iPSC)- derived astrocytes, which replicates several aspects of reactive astrocytes. We analyzed major events related to the time course of astrogliosis by measuring nuclear translocation of NF-kB, secretion of cytokines and changes in morphological phenotypes of human iPSC-derived astrocytes. These cells responded to TNF-α by promoting NF-kB nuclear translocation. Additionally, in ammation-related cytokines were found increased following TNF-α stimulation. Some of these cytokines showed increased gene expression according to qPCR measurements. Cells exposed to TNF-α also exhibited typical phenotypes of astrogliosis, such as an increase in vimentin and GFAP immunolabeling, changes in the cell aspect ratio and the shrinkage of nuclei. Moreover, a systematic decrease in d-[ H]aspartate uptake along the time course of astrogliosis was observed. Taken together, our results indicate that iPSC-derived astrocytes successfully reproduce major hallmarks of astrogliosis in culture and also con rm that the glial glutamate/aspartate uptake system is disrupted in human activated astrocytes. Thus, the model of human astrogliosis described here may contribute to better understand of amatory components of human neurological disorders and potentially serve as a tool for drug screening.
